哥本哈根大学

The Molecular Mechanisms of Polycystic Kidney Disease

项目介绍

This fully funded PhD student position is an opportunity to work in the Department of Biomedical Sciences, Heart and Kidney section, commencing 1 February 2022 or as soon as possible.

Our group and research
The supervisor team consists of a kidney physiologist specialising in kidney vasculature and in vivo models, and a kidney physiologist/medical doctor, specialising in kidney disease, cell models and kidney epithelial transport mechanisms. Both are associated with the Heart and Kidney section, which focuses on vascular, heart and kidney physiology.

Project description

Autosomal Dominant Polycystic Kidney Disease (ADPKD) is an inherited disease affecting 1:500-1000 live births. ADPKD is characterized by the development of large fluid-filled cysts in the kidneys that compromise the normal kidney tissue leading to end stage renal disease and major cardiovascular complications such as hypertension. A defect in kidney water transport is central to the disease, and the purpose of this project is to enhance our understanding of the intracellular regulation of the kidney collecting duct water channel aquaporin-2, to understand its role in ADPKD, and to investigate novel compounds for their potential to inhibit cyst progression and treat the concentrating defect.

The project is highly ambitious and will incorporate a wide range of in vivo and in vitro techniques. An available mouse model of ADPKD will be utilized to test a variety of drugs and we will use telemetry and ultrasound/MRI scanning to monitor blood pressure and disease progression. We will develop 3D cell culture models of in vitro cysts in order to directly study the role of water transport in cyst development. This will involve lentivirally transduced CRISPR/CAS9-mediated knockout of genes involved in cyst development and shRNA-mediated knockdown of aquaporin-2. We will also incorporate biochemical techniques to study protein membrane targeting in our in vitro cyst models. Basic laboratory techniques such as western blotting, PCR, immunostaining and confocal microscopy, are utilized in all aspects of the project. If time allows, we will embark upon a drug discovery aspect of the project involving large-scale study of protein-protein interactions and utilize compound library screening to identify small molecules that affect protein binding in ways that are of potential benefit in water transport regulation and ADPKD.

Principal supervisor is Associate Professor Charlotte Mehlin Sørensen, Department of Biomedical Sciences.

All inquiries regarding the position are to be directed to the co-supervisor: Assistant Professor Emma Tina Bisgaard Olesen, Department of Biomedical Sciences, Email etbo@sund.ku.dk, Direct Phone: +4524873391

Start:                Date: 1 February 2022 or as soon as possible

Duration:         3 years as a PhD student

Job description
Your key tasks as a PhD student at SUND are:

  • Carry through an independent research project under supervision
  • Use and develop novel research techniques

  • Complete PhD courses or other equivalent education corresponding to approx. 30 ECTS points
  • Participate in active research environments
  • Obtain experience with teaching or other types of dissemination related to your PhD project
  • Teach and disseminate your knowledge
  • Write a PhD thesis on the grounds of your project

Key criteria for the assessment of applicants
We are looking for a highly motivated, ambitious PhD-student who is interested in the field of kidney physiology and pathophysiology and who has the courage to learn to master a range of research techniques. Applicants must have qualifications corresponding to a master’s degree related to the subject area of the project e.g. molecular biomedicine, molecular biology, or medicine. Laboratory experience, especially with the standard techniques applied to the project (in particular cell studies, western blotting and PCR) is a great advantage.

Other important criteria are:

  • The grade point average achieved
  • Previous publications
  • Relevant work experience
  • Other professional activities
  • Curious mind-set with a strong interest in cell biology and kidney (patho)physiology
  • English language skills

Place of employment

The place of employment is at the Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen. We offer creative and stimulating working conditions in dynamic and international research environment. Our research facilities include modern laboratories and a strong national and international network of collaborators.

Terms of employment
The employment as PhD fellow is full time and for 3 years.

It is conditioned upon the applicant’s success­ful enrolment as a PhD student at the Graduate School at the Faculty of Health and Medical Sciences, University of Copenhagen. This requires submission and acceptance of an application for the specific project formulated by the applicant.

The PhD study must be completed in accordance with The Ministerial Order on the PhD programme (2013) and the Faculty’s rules on achieving the degree. Salary, pension and terms of employment are in accordance with the agree­ment between the Ministry of Finance and The Danish Confederation of Professional Associations on Academics in the State. Depending on seniority, the monthly salary begins around 27.871 DKK /approx. 3.745 EUR (October 2021-level) plus pension.

Questions
For specific information about the PhD fellowship, please contact Assistant Professor Emma Tina Bisgaard Olesen, Department of Biomedical Sciences, Email etbo@sund.ku.dk, Direct Phone: +4524873391.

General information about PhD study at the Faculty of Health and Medical Sciences is available at the Graduate School’s website: https://healthsciences.ku.dk/phd/guidelines/

Application procedure
Your application must be submitted electronically by clicking ‘Apply now’ below. The application must include the following documents in PDF format: 

1. Motivated letter of application (max. one page)

2. CV incl. education, experience, language skills and other skills relevant for the position

3. Master of Science diploma and transcript of records. If not completed, a certified/signed copy of a recent transcript of records or a written statement from the institution or supervisor will do

4. Publication list (if possible)

5. References from previous supervisor(s)/employer(s) if possible

Application deadline: 7 November 2021 23.59pm CET

We reserve the right not to consider material received after the deadline, and not to consider applications that do not live up to the abovementioned requirements.

The further process
After the expiry of the deadline for applications, the authorized recruitment manager selects applicants for assessment on the advice of the hiring committee. All applicants are then immediately notified whether their application has been passed for assessment by an unbiased assessor.

The assessor makes a non-prioritized assessment of the academic qualifications and experience with respect to the above-mentioned area of research, techniques, skills and other requirements listed in the advertisement.

Once the assessment work has been completed each applicant has the opportunity to comment on the part of the assessment that relates to the applicant him/herself.

You can read about the recruitment process at http://employment.ku.dk/faculty/recruitment-process/

The applicant will be assessed according to the Ministerial Order no. 242 of 13 March 2012 on the Appointment of Academic Staff at Universities.

Interviews are expected to be held in week 48 or soon thereafter.

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截止日期 2021-11-07
哥本哈根大学

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哥本哈根大学坐落于丹麦王国首都哥本哈根,是丹麦最高学府,国际研究型大学联盟和欧洲研究型大学联盟成员。
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联系方式

电话: +45 35 32 26 26

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