项目介绍

2025年12月4日

Join us in unlocking the secrets of signal integration in plants!

Plants must cope with a wide diversity of stresses such as drought, soil salinisation and disease. Their survival hinges on the continuously perception, integration and interpretation of an immense number of signals from the environment and generating the appropriate responses. They must deal with this on the spot. Millions of years of evolution have allowed plants to adapt to almost every climate and has made them to masters perceiving and responding to countless signals. While traditional approaches have uncovered much of the genetic foundation of response pathways to individual signals, how plants integrate multiple co-occurring signals on the cellular level has remained largely unexplored.

What you will do

We are looking for a dedicated PhD-candidate to join our team, who can demonstrate initiative in driving their work forward. Together, we will unlock this new dimension of signalling in plants by deciphering the mechanisms that enable plants to respond rapidly and in a coordinated manner to environmental challenges.

The position is supported by a recently awarded NWO VIDI grant and is situated in the research team of Dr. André Kuhn. His team is embedded within the Plant Cell Biology group that is part of the Green Life Sciences theme at the Swammerdam Institute for Life Sciences.

This project aims to uncover (1) cellular processes regulated by multiple co-occurring signals, (2) molecular components of signal integration pathways, and (3) the molecular mechanisms of how these components work together to govern dynamic regulation of specific cellular processes. Our innovative approach combines proteomics, biochemistry, and advanced imaging methodologies to delineate the early phases (seconds to minutes) of plant signal integration, using Arabidopsis thaliana as a model plant. 

You will identify proteins that are rapidly phosphorylated upon co-occurring signals using phospho-proteomics. Proteins that are potential hubs of signal integration will be characterized through molecular- and biochemical methods. Using microfluidics coupled with advanced imaging methods, you will systematically test which cellular processes are dynamically regulated and investigate how these processes connect to plant growth and resilience. 

Tasks and responsibilities: 

• complete PhD thesis within official appointment duration (four years);
• systematically and independently design, perform and document experiments;

• be an active and responsible member of our research group;
• discuss work with group members and during departmental meetings to incorporate feedback;

• take a leading role in writing manuscripts for publication in peer-reviewed journals;
• participate in PhD training programme of the University of Amsterdam and the national graduate school Experimental Plant Sciences (EPS);
• assist teaching and supervision of Bachelor and Master students.

You will get the opportunity to:

• present results at (inter)national scientific conferences;
• expand academic, professional, and personal skills;
• contribute to science communication and outreach activities;
• thrive in a team that values impactful research and mutual support;
• combine the advantages that a collaborative project offers, such as access to state-of-the art research facilities at the Swammerdam Institute and UvA.

Your profile

You are passionate about science and have a particular interest in cell biology and biochemistry. You enjoy performing experimental lab work (e.g. molecular biology, plant transformations, (phospho)proteomics), as well as bio-imaging and data analysis using bioinformatics tools. You have a creative mind and look forward to learning and expanding your skills. Finally, you are a team player and a pleasant colleague who enjoys being part of an international team of scientists.

Your experience:

• MSc degree in molecular biology or biochemistry;
• demonstrated hands-on experience with some of the following techniques: protein biochemistry, (phospho)proteomics, molecular cloning, confocal imaging and plant growth assays;
• fluent in English, both written and spoken.

It is a preference if you additionally have experience in microfluidics, proteomics and/or image data analysis (e.g. using Python or R). 

What we offer you

A temporary contract for 38 hours per week for the duration of 4 years (the initial contract will be for a period of 18 months and after satisfactory evaluation it will be extended for a total duration of 4 years). The preferred starting date is no later than May 1st. This should lead to a dissertation (PhD thesis). We will draft an educational plan that includes attendance of courses and (international) meetings. We also expect you to assist in teaching undergraduates and master students.

Based on a full-time appointment (38 hours per week) the gross monthly salary will range from €3.059 in the first year to €3.881 (scale P) in the last year. This does not include 8% holiday allowance and 8,3% year-end allowance. The UFO profile PhD candidate is applicable. A favourable tax agreement, the ‘30% ruling’, may apply to non-Dutch applicants. The Collective Labour Agreement of Universities of the Netherlands is applicable.

Besides the salary and a vibrant and challenging environment at Amsterdam Science Park we offer you multiple fringe benefits:

• 232 holiday hours per year (based on fulltime);
• multiple courses to follow from our Teaching and Learning Centre;
• a complete educational program for PhD students;
• a pension at ABP for which UvA pays two third part of the contribution;
• the possibility to follow courses to learn Dutch;
• help with housing for a studio or small apartment when you’re moving from abroad.

Are you curious to read more about our extensive package of secondary employment benefits, take a look here.