苏黎世联邦理工

Cellular structural biology and basic medicine of motile cilia

项目介绍

The position is to be a PhD student of D-BIOL/ETHZ. The workplace is the group of Prof. Takashi Ishikawa at Paul Scherrer Institute (you are employed by PSI).

Project background

The positions are funded by Swiss National Science Foundation and Personalized Health and Related Technologies.

Job description

Motile cilia are appendage-like organelles, which make beating motion to cause flow of extracellular fluids or enable cells to swim. Motile cilia are made of more than 400 proteins and function in sperm, the oviduct, brain, tracheae and the embryo. Its defect results in respiratory diseases, mental disorder, male and female infertility, and developmental abnormality, called ciliopathy.

My group has been investigating the molecular mechanism of beating of cilia, employing cryo-electron microscopy (summarized in the review article, Ishikawa (2017) Cold Spring Harb Perspect Biol. 9(1):a028076. doi: 10.1101/cshperspect.a028076.)). Now we expand our research field by combining cryo-EM with cross-linking mass-spectrometry, collaborating with Dr. Alexander Leitner (D-BIOL/ETHZ), which is the focus of one PhD project. With this project, the student can learn both cryo-EM and MS using model organisms, such as unicellular algae Chlamydomonas, to investigate 3D conformation and arrangement of component proteins in cilia.

The other project is to study human ciliopathy using cultured ciliated epithelial cells and cryo-EM. We succeeded to culture human bronchial epithelial cells and image cilia from them by cryo-EM, especially cryo-electron tomography (cryo-ET). Our collaborator, ciliopathy experts at Inselspital (Bern), will provide cells from ciliopathy patients. We will culture them and analyze cilia, preparing by cryo-ultramicrotomy (at Univ. Bern) and cryo-FIB (ETH Zurich) milling method. The PhD student will pursue molecular mechanism of ciliopathy and can also learn cryo-EM, human cell culture and various cell biology methods.

For both projects, high-end cryo-EMs are available at ETH Zurich and PSI. Cell biology, biochemistry and computational facilities are located at PSI.

Your profile

MSc degree in the field of biology, biochemistry with experience of protein biochemistry, cell culture

or

MSc degree in the field of physics, informational science (image processing/analysis oriented) with keen interest in structural biology and biological imaging

Experience of genetic engineering and computation (script writing etc) will be highly welcomed but not necessary.

Interested?

We look forward to receiving your online application.

Please note that we exclusively accept applications submitted through our online application portal. Applications via email or postal services will not be considered.

Further information about the Paul – Scherrer – Institute can be found on our website. Questions regarding the position should be directed by email to takashi.ishikawa@psi.ch (no applications).

录取要求

  • MSc degree in the field of biology, biochemistry with experience of protein biochemistry, cell culture OR MSc degree in the field of physics, informational science (image processing/analysis oriented) with keen interest in structural biology and biological imaging
  • Experience of genetic engineering and computation (script writing etc) will be highly welcomed but not necessary.

项目概览

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欧陆, 瑞士 所在地点
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截止日期 2021-10-10
苏黎世联邦理工

院校简介

苏黎世联邦理工是国际研究型大学联盟、全球大学高研院联盟、IDEA联盟成员,是闻名全球的世界顶尖研究型大学,连续多年位居欧洲大陆高校翘首。
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电话: +41 44 632 11 11

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